Anemia, a hematological disease in which the hemoglobin concentration is lower than normal, is a prevalent condition that can develop regardless of age. Approximately 800 million women and children worldwide were affected by anemia in 2011, according to WHO. The study aimed to assess the knowledge and awareness level of anemia symptoms, types, and risks among the Saudi Arabian population. This observational cross-sectional study was carried out using a self-administered questionnaire during the period from July 2022 to May 2023 across various regions of Saudi Arabia. A total of 1003 participants from different cities in Saudi Arabia will take part in the study. Data was encoded in Microsoft Excel files before being exported into SPSS program (version 26) for analysis. All tests utilized in the study will have a significance level of 0.05. The study included 1003 participants; most of them, 76.6%, were females and 23.4% males. 41.2% of participants aged 18-24 years. Nearly half of the participants, 49.5%, reported knowledge above average score, followed by 27.1% with an excellent score, 23.3% middle score, and only 0.1% reported a poor score about anima knowledge. Based on the findings of the current study, it could be concluded that participants had adequate general knowledge about anemia and a good knowledge score on anemia. It generally corresponded with reported figures from other parts of the world. Our findings indicate a substantial relationship between knowledge score, gender, and age.