The development and metabolism of tomato plants are influenced by climate and genetic diversity. However, it is still infrequently investigated how these two factors affect it. As a result, this study demonstrates that the lycopene concentration of tomato plants is affected by the environment and variances in tomato plant types. The Gustavo Cultivar and the Ros Cultivar of tomato plants were used in this study. The two types came from two separate climate zones in Central Sulawesi Province, Indonesia: the Napu Region and the Sigi Region. The Napu region has a temperate climate, while the Sigi region enjoys hot weather. Tomatoes from two distinct types and areas were then removed and tested for lycopene concentration with a 472 nm UV-Vis spectrophotometer. The results reveal that temperature and cultivar changes have a substantial effect on the lycopene concentration of the Ros Cultivar tomatoes. This climate variation, however, did not occur in the Gustavo Cultivar. The Ros Cultivar had the highest lycopene content. The warmer temperature raised the level of lycopene in the Ros Cultivar but not in the Gustavo Cultivar.