The present research determined how the anticoagulant drug Heparin caused osteoporosis in rats and how Taraxacum officinale and/or vitamins can prevent it (C, K). Fifty female Wester albino rats were used in the 30-day study. They were placed into five groups. In the heparin-treated group, bone-specific alkaline phosphatase (ALP), osteocalcin (CO), deoxypyridinoline (DPD), and tartrate-resistant acid phosphatase (TRAP) were significantly increased in contrast to the control group. Taraxacum officinale and/or Vitamins (C, K) co-treatment of rats receiving heparin significantly reversed the declines in the examined parameters and the histomorphologic bone tissue images. In conclusion, the data showed that Taraxacum officinale and /or Vitamins (C, K) could protect the bone tissues from the damaging impact of heparin, which was more pronounced in rats treated with the combination of the two agents Taraxacum officinale and Vitamins (C, K). The current study may support the utilization of this combination as a promising therapy to alleviate the adverse of the anticoagulant heparin.