The negative effect of vibration, as the main physical production factor, should be thoroughly investigated. In this scientific work, the effect of general vibration on protein metabolism in the body is studied using the example of laboratory animals. 2 series of experiments were conducted on 30 white rats kept in the same conditions. At the same time, animals of group 1 were exposed to vibration, unlike animals of group 2. Animals of group 1 were exposed to a general vertical sinusoidal vibration with a frequency of 20 Hz with a vibration velocity of 126 dB for 4 hours daily for 8 weeks. The results of the studies did not reveal significant changes in the total protein content. However, there was a significant decrease in albumin in the content of protein fractions, as well as an increase in the fractions of α- and γ-globulins. It was found that by the end of the experiment, there was a decrease in the content of total amino acids in the blood serum of experimental animals: aspartic acid (P