Job satisfaction is a key indicator to evaluate health-care quality. Poor job satisfaction has been linked to poor healthcare delivery and also psychological problems have been associated with health-care professionals. The study assessed Nigerian hospital pharmacists’ job satisfaction and factors that influence satisfaction with the job. A cross-sectional survey was conducted among consenting hospital pharmacists in Nigeria using a validated self-administered “American Hospital Pharmacist Job Satisfaction Survey” (AHPJSS) questionnaire. The data was collected from September 2019 to March 2020. To summarize the data, descriptive and inferential statistics were employed. SPSS version 23 was used to analyze the data.  The statistical significance level was set at p ≤ 0.05. Of the 572 respondents (response rate = 71.5%), 33.7 % of the hospital pharmacists were between 30 to 39 years of age and most of the pharmacists were male (51.0 %). Approximately 52% of Nigerian hospital pharmacists had job satisfaction. Job satisfaction was substantially related to age (X2(4) = 13.56, p = 0.009) and working experiences (X2 (1) = 6.70, p = 0.010). The intrinsic factors that influenced job satisfaction among Nigerian hospital pharmacists were recognition from peers and the challenge of the job and extrinsic factors that influenced job satisfaction among Nigerian hospital pharmacists were staffing of the pharmacy department and feedback from supervisors. Most of the Nigerian hospital pharmacists were satisfied with their jobs. Predictors of job satisfaction were; recognition from co-workers, challenges the job provides, staffing, recognition from other co-workers, salary, and feedback from supervisor(s).