Laurus nobilis is used as medicine and food additives in Saudi Arabia, and also, it could be used to study as antibacterial activity and assessment through agar well diffusion and most susceptible bacteria used was Pseudomonas aeruginosa, flowed by Staphylococcus aureus. Resistant bacteria mostly were Bacillus subtilis followed by MRSA while there was no effect on Micrococcus luteus. HPLC analysis found the presence of 11 phenolic compounds isolated from Laurus nobilisincluding Gallic Acid, Catechin, Coffeic Acid, Syringic Acid, Rutin, Coumaric Acid, Naringenin, Quercetin, Ferulic Acid, Vanillin, and Cinnamic Acid. The result showed that obtained found variations from the concentration ratio of ethanolic Laurus nobilis leaves extract. HPLC illustrated that Rutin, Gallaic acid, and syringic acid in high concentrations were 418.37, 81.66, and 34.43 µg/g, respectively and phenolic compounds ranged from 0.21 to 21.42 µg/g and the lowest Cinnamic concentration was 0.21µg/g.