Association between thrombophilic gene polymorphisms and recurrent pregnancy loss in Iranian Women

Background: Nearly one in five pregnancies ends in abortion. The recurrent spontaneous abortion (RSA) occurs in about 2% of couples for various reasons. Thrombophilia is considered as a major risk factor in women with RSA. This study analyzed seven polymorphisms of five common genes in thrombophilia and their correlations with RSA in Iranian women. Material and Methods: This comparative, observational (case-control) study was conducted on 60 women with history of RSA and 60 healthy women with successful pregnancies who referred to Obstetrics and Gynecology Department of Eghbal Hospital (Tehran, Iran) within the 2018-2019 period. The amplification-refractory mutation system (ARMS) PCR technique was used to analyze polymorphisms of FV Leiden (G1691A), PAI-1 (-844 A/G), PAI-1 (-675 4G/5G), β-Fibrinogen (-455 G/A), MTHFR (C677T), MTHFR (A1298C), and Prothrombin (G20210A). Results: There was a significant difference between the two study groups in the genotypes of MTHFR (C677T) and PAI-1 (-844A/G) (p value=0.03 and 0.00, respectively). Conclusion: According to the research data, analysis of polymorphism of MTHFR (C677T) and PAI-1 (-844 A/G) can be used as a good marker to address the probable causes RSA and a significant prognostic factor for consecutive pregnancy losses.