A naturally occurring gas made of three oxygen atoms is called ozone. It has strong antibacterial properties and a massive oxidizing effect on oral infections. This study aimed to assess the knowledge and awareness level of the usage of Ozone therapy on periodontal health among dental interns, General dental practitioners, and specialist in KSA. A cross-sectional survey was conducted in Saudi Arabia. The study was among dental interns, General dental practitioners, and professionals, during the period from January -December 2022. An online questionnaire was done to test the knowledge and attitude of dental interns and undergraduate students about the use of ozone therapy on periodontal health used in data collection. All data were collected and analyzed by SPSS software, and the results of the participant answers were represented as pie charts and graphs. The study included 413 participants, 58.1% of them were females and 41.9% were males. 82.9% aged between 24- 33 years old. 51.6% of participants heard of ozone therapy. 82.3% were interested to read about the usage of ozone therapy in dentistry. 45.5% of participants believe in the efficiency of ozone therapy. 36.3% of participants had good knowledge, 38.7% had moderate knowledge and 24.9% had poor knowledge. In conclusion, Saudi dentists had moderate to poor knowledge of periodontal ozone therapy. Knowledge score was significantly associated with age but not with gender or clinical experience.
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