There is the various disease which is caused by contaminated water. Unfortunately, only 40-60% Pakistani population has access to safe water. This raises a serious issue to perform bacteriological analysis of drinking water. Although such analysis was carried on by some laboratories but the method or techniques adopted were very old and also there is no database to evaluate the actual figure. The study was cross-sectional. In the present study, the bacteriological quality of the drinking water supply was analyzed. Different districts of Karachi were selected for the study. The quality of drinking water in both rural and urban areas is not properly accomplished resulting in a higher occurrence rate of water-borne diseases. Pathogenic organisms cause water-borne diseases. Water samples from four different districts were collected. A total of 115 water samples were assessed for bacteriological contamination. The result showed that 55 out of 115 (47.8%) did not comply with the World Health Organization(WHO) standard guidelines for drinking water. Escherichia coli was identified among 23 out of 55 samples (41.8%) and overall coliform organisms were grown from 63.3%. It is a matter of great concern that 47.8% of samples were sub-standard and unsatisfactory to be consumed by humans. It requires serious strategies to cope up with the issue.
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