Infertility is one of the developing problems in the most countries and it has a lot of circumstance problems, includes, emotional, social and political. About half of infertilites are due to male factor However, effective treatment for male-factor infertility was not available until 1992 ,when intracytoplasmic sperm injection (ICSI) The overall success rate of ICSI depend on many factor such as ,The age of couples ,influence of controlled ovarian hyper stimulation (COH) , timing and number of insemination, and duration and cause of infertility In clinical practice ,the manual-visual light microscopic methods for evaluating semen quality maintain their central role in assessment of male fertility potential The semen analysis such as count,motility and morphology have been reported to be associated with a favorable outcome in ICSI methods, mainly considered as laboratory techniques for improving the quality of sperm, include Swim-Up (SU) and Density Gradient Centrifugation (DGC). The SU is a common technique in IVF labs, and is mainly performed in a sample of semen having normal sperm concentration. In this technique, sperms are selected based on their motility and their capacity to leave the semen plasma. In the DGC method, sperms are selected based on the density, motile sperm are separated from dead sperms, leukocytes and other high-density semen plasmatic compounds. The aim of this method is thus to select sperms with high motility and morphology rates .so the aim of the present study is to compare the effect of these two methods on fertilized oocytes ,transfer embryo, formation of blastocyte rate(BR) ,grading of embryo and on the outcome in ICSI include of, pregnancy rate (PR), abrotion rate(AR). in different groups. This groups inaccording to samples are choosed for ICSI, including, oligospermia and asthenospermia, Teratosphermia and Oligoastenotrotoospermia. Our results revealed the effectiveness of the SU technique compared to DGC-swin up as a sperm preparation method with a favorable ICSI success, The present study not only demonstrates the greater reproducibility of the results with SU when compared with the DGC, but what stands out is the possibility to use a non-invasive method on semen treatment whit statistically better results for embryo development and pregnancy. One explanation for this could be found in the better health status of the embryo obtained from spermatozoa that have suffered less stress during the treatment.
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