Empirical studies on the effect of ECM matrix derived decellularized bovine mesentery on the behavior of Blastema cells in vitro

Objective (s): Extracellular matrix, in addition to its physical role can also control cell behaviors such as cell proliferation differentiation and migration using its inductive effects. In this study, extracellular matrix derived from bovine mesentery as a three-dimensional scaffold was used to study the behavior of blastema cells. Materials and Methods: bovine mesentery, cut into pieces of 5 mm by 5 mm was decellularized by combination of physical and chemical methods including snap freeze‐thaw and  treatment with sodium dodecyl sulfate (SDS). These parts were assembled as scaffolds inside the blastema rings from rabbits pinna. Results: Histological studies and scanning electron microscopy showed the removal of cells while preserving matrix of epithelium and collagen fibers in mesenteric tissue. Furthermore adhesion, polarity, migration and maintenance of blastema cells was observed on mesenteric decellularized scaffolds. Also, in the third week of culture their differentiation to mesothelium-like structures and the establishment of tight junction between these cells as well as differentiation of fibroblasts and adipocytes were observed within the mesenteric scaffold. Conclusion: The results of the morphological studies of this study indicate that mesenteric decellularized ECM can have inductive effects on the migration and placement of inflammatory tissue cells and possibly to differentiate them into plain squamous mesothelioma cells, fibroblasts and adipocytes. Although more studies are needed to prove the identity of the cells and other features of the scaffold.