The Effect of Different Levels of Flaxseed Oil on Biochemical Changes in Hypercholesterolemic Rats

Flaxseed oil has various benefits over multiple chronic diseases in humans and animals. Besides, flaxseed oil has shown the potential to avert lipid disorders. However, various researches have indicated that lower serum cholesterol in animals and human beings can be attributed to whole flaxseed oil. Therefore, this study aimed at determining the effect of different levels of flaxseed oil on biochemical changes in hypercholesterolemic rats. In this study, (24) albino mice were used and divided into two main groups. The first was a group of mice infected with hypercholesterolemia, the second negative control group was a group of non-infected mice. They were then divided into four groups with two of them given different concentrations of flaxseed oil (20% and 25%). Of the other two groups, one positive infected and another uninfected negative control group were not kept on the experimental diet meaning that all mice were divided into 4 groups consisting of 6 mice in each group. The result showed that non-significant differences in HDL levels between 20%, 25% flaxseed oil and control negative when compared with control positive Also, there were significant decreases in ALP between the groups replaced with different percentage of flaxseed oil compared with control (+) group. The results suggest to use different levels of flaxseed oil for hypercholesterolmic patients, also suggests future studies may be to evaluate the efficacy and advantage of using flaxseed oil as extracts.

1.        Goyal A, Sharma V, Upadhyay N, Gill S, Sihag M. Flax and flaxseed oil: an ancient medicine & modern functional food. J Food Sci Technol. 2014;51(9):1633-53.

2.        Tanideh R, Delavari S, Farshad O, Irajie C, Javad Yavari Barhaghtalab M, Koohpeyma F, et al. Effect of flaxseed oil on biochemical parameters, hormonal indexes and stereological changes in ovariectomized rats. Vet Med Sci. 2021;7(2):521-33.

3.        Shahidi F, Ambigaipalan P. Omega-3 polyunsaturated fatty acids and their health benefits. Annu Rev Food Sci Technol. 2018;9:345-81.

4.        Tavarini S, Castagna A, Conte G, Foschi L, Sanmartin C, Incrocci L, et al. Evaluation of chemical composition of two linseed varieties as sources of health-beneficial substances. Molecules. 2019;24(20):3729.

5.        Rangrej V, Shah V, Patel J, Ganorkar PM. Effect of shortening replacement with flaxseed oil on physical, sensory, fatty acid and storage characteristics of cookies. J Food Sci Technol. 2015;52(6):3694-700.

6.        Omar AM. The potential protective influence of flaxseed oil against renal toxicity induced by thioacetamide in rats. Saudi J Biol Sci. 2018;25(8):1696-702. doi:10.1016/j.sjbs.2016.09.021

7.        Moneim AE, Dkhil MA, Al-Quraishy S. The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater. 2011;194:250-5. doi:10.1016/j.jhazm at.2011.07.097

8.        Campbell JA. Methodology of protein evaluation. RAG Nutr. Document R. 1963;10.

9.        Rashwan NM. Special Diet for Inducing Hypercholesterolemia (Doctoral dissertation, Ph. D. Thesis, Nutrition and Food Science Dep. Faculty of Home Economics Helwan University).

10.      Chapman DG, Castillo R, Campbell JA. Evaluation of protein in foods: 1. A method for the determination of protein efficiency ratios. Can J Biochem Physiol. 1959;37(5):679-86.

11.      Trinder P. Glucose & triglycerides enzymatic colorimetric methods. J Ann Clin Biochem. 1969;6:24-7.

12.      Fassati P, Prencipe L. Triglyceride enzymatic colorimetric method. J Clin Chem. 1982;28:2077.

13.      Allain CC. Cholesterol enzymatic colorimetric method. J Clin. Chem. 1974;20:470.

14.      Lopez MF. HDL-cholesterol colorimetric method. J Clin Chem. 1977;23:882.

15.      Tietz NW. Fundamental of Clinical Chemistry. 2th ed. Philadelphia, W.B; 1976. pp.53-6. 

16.      Henry RJ. Creatinine Colorimetric Method. Principles of Techniquss, 2nd Ed., Harcer and Row Pub; 1974. p.525.

17.      Patton CJ. Urea enzymatic method. J Anal Chem. 1977;49:464-546.

18.      Yari Z, Naser-Nakhaee Z, Karimi‐Shahrbabak E, Cheraghpour M, Hedayati M, Mohaghegh SM, et al. Combination therapy of flaxseed and hesperidin enhances the effectiveness of lifestyle modification in cardiovascular risk control in prediabetes: a randomized controlled trial. Diabetol Metab Syndr. 2021;13(1):3. doi:10.1186/s13098-020-00619-y

19.      Vijaimohan K, Jainu M, Sabitha KE, Subramaniyam S, Anandhan C, Devi CS. Beneficial effects of alpha linolenic acid rich flaxseed oil on growth performance and hepatic cholesterol metabolism in high fat diet fed rats. Life Sci. 2006;79(5):448-54.

20.      Brizzi P, Tonolo G, Carusillo F, Malaguarnera M, Maioli M, Musumeci S. Plasma lipid composition and LDL oxidation. Clin. Chem Lab Med. 2003;41(1):56-60.

21.      Omar AM. The potential protective influence of flaxseed oil against renal toxicity induced by thioacetamide in rats. Saudi J Biol Sci. 2018;25(8):1696-702.

22.      Bhatia AL, Sharma A, Patni S, Sharma AL. Prophylactic effect of flaxseed oil against radiation‐induced hepatotoxicity in mice. Phytother Res. 2007;21(9):852-9.

23.      EL-BAHY AM, El-Bendary S, El-Megeid AA, Mahmoud E, Shoib YF, Hashem TM. Effect of flax seeds, pumpkin seeds and sesame seeds on liver enzymes and kidney functions of rats suffering from osteoporosis. New Egypt J Med [The]. 2011;45(5):404-14.