Archive \ Volume.14 2023 Issue 2

Effect of Moringa on Biochemical and Immunological Changes in Hyperglycemic Rats

Magbolah Salem Helal Alzahrani

Phytotherapy is the treatment and prevention of diseases using plants, plants part such as herbal, flowers, leaves, peels, and roots or mixtures of them. This study seeks to determine the impact of moringa on biochemical and immunological alterations in hyperglycemic rats. Moringa is one of the most herbal plants with remarkable medicinal effects, including treating high blood sugar and enhancing immune functions. Twenty-four (24) male albino Sprague-Dawley strain rats were separated into four groups, with one serving as the negative control group (normal); the other groups were used to induce diabetes with alloxan. The rats were ten weeks old and weighed 15010g. Two of the diabetes groups received 5% 10% Moringa for 28 days, while the positive control group of one was given a conventional diet. After the experiment, a blood sample was obtained, and the organs were taken out and biochemically analyzed. Therefore, when compared to the control (+) group, all experimental groups exhibited significantly different glucose levels. In addition, when compared to the control (+) group, group 4 (10% Moringa) had the best effects on the triglycerides, HDL, and LDL of the diabetic rats. In diabetic rats, ALP, AST, ALT, and uric acid were best measured in Group 3 (5% Moringa), while urea and creatinine were best measured in Group 4 (10% Moringa). Even when measured against the reference (+) group. This study suggested giving diabetics varying doses of moringa, as well as perhaps giving them varied doses of moringa to lower LDL and atherogenic index readings.

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How to cite:
Alzahrani MSH. Effect of Moringa on Biochemical and Immunological Changes in Hyperglycemic Rats. Arch Pharm Pract. 2023;14(2):30-6.
Alzahrani, M. S. H. (2023). Effect of Moringa on Biochemical and Immunological Changes in Hyperglycemic Rats. Archives of Pharmacy Practice, 14(2), 30-36.

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1.        Arnarson A. Science - Based Health Benefits of Moringa oleifera. 2018. Available from:

2.        Mun’im A, Puteri MU, Sari SP. Anti-anemia effect of standardized extract of Moringa oleifera Lamk. Leaves on aniline induced rats. Pharmacogn J. 2016;8(3).

3.        Mahdi HJ, Khan NA, Asmawi MZ, Mahmud R, Vikneswaran A, Murugaiyah L. In vivo anti-arthritic and anti-nociceptive effects of ethanol extract of Moringa oleifera leaves on complete Freund's adjuvant (CFA)-induced arthritis in rats. Integr Med Res. 2018;7(1):85-94. doi:10.1016/j.imr.2017.11.002

4.        Bakre AG, Aderibigbe AO, Ademowo OG. Studies on neuropharmacological profile of ethanol extract of Moringa oleifera leaves in mice. J Ethnopharmacol. 2013;149(3):783-9.

5.        Aleksic A. Top 22 Moringa Health Benefits Side Effects. 2019. Available from:

6.        Maurya SK, Singh AK. Clinical efficacy of Moringa oleifera Lam. stems bark in urinary tract infections. Int Sch Res Notices. 2014;2014.

7.        Centers for Disease Control and Prevention. National Diabetes Statistics Report, 2017: Centers for Disease Control and Prevention, US Department of Health and Human Services. Available from: External link

8.        Kannur DM, Hukkeri VI, Akki KS. Antidiabetic activity of Caesalpinia bonducella seed extracts in rats. Fitoterapia. 2006;77(7-8):546-9. doi:10.1016/j.fitote.2006.06.013

9.        Morsi AE. Your Health and Healing between your Hands in Herbs. Arabic, Egypt. 1992.

10.      Hegsted D, Mills R, Perkins E. Salt mixture. J Biol Chem. 1941;138:459.

11.      Campbell JA. Methodology of Protein Evaluation. RAG Nutr. Document R. 37. June Meeting New York. Chapman, DG. 1963:679-86.

12.      Trinder P. Glucose enzymatic colorimetric method. J Clin Biochem. 1969;(6):24.

13.      Fassati P, Prencipe L. Triglyceride enzymatic colorimetric method.  J Clin Chem. 1982;28:2077.

14.      Allain CC. Cholesterol enzymatic colorimetric method. J Clin Chem. 1974;20:470.

15.      Lopez MF. HDL-cholesterol colorimetric method. J Clin Chem. 1977;23:882.

16.      Schmit JM. Colorimetric determination of total lipids using sulfophosphovanilic mixture. Lyon, France: BioMerieux Company of France. 1964.

17.      Tietz NW. Fundamental of Clinical Chemistry. Philadelphia. 1976;(2):53-6. 

18.      Henry RJ. Clinical Chemist: Principles and Technics, 2nd Edition, Hagerstown (MD), Harcer, Row. 1974;882.

19.      Patton CJ, Crouch SR. Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal Chem. 1977;49(3):464-9. doi:10.1021/ac50011a034

20.      Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst. 1972;97(1151):142-5.

21.      Faulkner WR, King JW. Fundamentals of clinical chemistry. Saunders WB, Philadelphia. 1976:994-8.

22.      Snedecor GW, Cochran WG. Statistical methods 6th edition. The Iowa State University. 1967.

23.      Bedosky L. What Are the Potential Health Benefits of Moringa Powder?. 2019. Available from:; benefits - disease all prevention weight - loss 8A D8 A9.

24.      Gonzalez Stuart A.  Moringa. 2020. Available from:

25.      Kushwaha S, Chawla P, Kochhar A. Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women. J Food Sci Technol. 2014;51:3464-9.

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