Archive \ Volume.15 2024 Issue 3

HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba

Marwa Melliti , Esraa Mohamed Musa , Fethia Skhiri , Hayet Edziri*✉
  1. Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000, University of Monastir, Tunisia.
  2. Department of Chemistry, Faculty of Science & Arts at Sharurah, Najran University, Saudi Arabia.
  3. Laboratory of Biodiversity Higher, institute of Biotechnology of Monastir, 5000, Tunisia.

Abstract

Pancratium maritimum is a species of bulbous plant that belongs to the Amaryllidaceae family, it is among one of the most important alkaloid-containing plant families. This study aimed to investigate the antioxidant, antibacterial, and antifungal activities of Pancratium maritimum extracts and their UHPLC-MS analysis. The total phenolic, tannins, and flavonoid content of Tunisian Pancratium maritimum aerial part methanolic extract were determined by colorimetric method. The phytochemicals in P.maritimum methanolic were identified using the UHPLC-MS method. The antioxidant activity was assessed using a DPPH assay. The antibacterial and antifungal properties were evaluated against a diverse array of pertinent pathogens using the broth dilution technique.

This study showed the presence of alcaloids in methanolic extract as hordenine, lycorine and galanthamine, a strong antioxidant activity was recorded for P.maritimum methanolic extract with a DPPH inhibition of (86.7%). The alkaloid fraction exhibited the most potent antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis bacteria, with minimum inhibitory concentration (MIC) values ranging from 1.125 to 2 mg/mL. Furthermore, it demonstrated significant anticandidal activity against Candida glabrata and Candida parapsilosis, with MIC values ranging from 0.031 to 0.125 mg/mL. P.maritimum extracts have the potential to be utilized for controlling bacterial biofilms in food and food-related situations.


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Vancouver
Melliti M, Musa EM, Skhiri F, Edziri H. HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba. Arch Pharm Pract. 2024;15(3):43-8. https://doi.org/10.51847/TnMvSYHLoO
APA
Melliti, M., Musa, E. M., Skhiri, F., & Edziri, H. (2024). HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba. Archives of Pharmacy Practice, 15(3), 43-48. https://doi.org/10.51847/TnMvSYHLoO

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