Archive \ Volume.15 2024 Issue 3

HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba

Marwa Melliti , Esraa Mohamed Musa , Fethia Skhiri , Hayet Edziri*✉
  1. Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000, University of Monastir, Tunisia.
  2. Department of Chemistry, Faculty of Science & Arts at Sharurah, Najran University, Saudi Arabia.
  3. Laboratory of Biodiversity Higher, institute of Biotechnology of Monastir, 5000, Tunisia.

Abstract

Pancratium maritimum is a species of bulbous plant that belongs to the Amaryllidaceae family, it is among one of the most important alkaloid-containing plant families. This study aimed to investigate the antioxidant, antibacterial, and antifungal activities of Pancratium maritimum extracts and their UHPLC-MS analysis. The total phenolic, tannins, and flavonoid content of Tunisian Pancratium maritimum aerial part methanolic extract were determined by colorimetric method. The phytochemicals in P.maritimum methanolic were identified using the UHPLC-MS method. The antioxidant activity was assessed using a DPPH assay. The antibacterial and antifungal properties were evaluated against a diverse array of pertinent pathogens using the broth dilution technique.

This study showed the presence of alcaloids in methanolic extract as hordenine, lycorine and galanthamine, a strong antioxidant activity was recorded for P.maritimum methanolic extract with a DPPH inhibition of (86.7%). The alkaloid fraction exhibited the most potent antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis bacteria, with minimum inhibitory concentration (MIC) values ranging from 1.125 to 2 mg/mL. Furthermore, it demonstrated significant anticandidal activity against Candida glabrata and Candida parapsilosis, with MIC values ranging from 0.031 to 0.125 mg/mL. P.maritimum extracts have the potential to be utilized for controlling bacterial biofilms in food and food-related situations.


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Vancouver
Melliti M, Musa EM, Skhiri F, Edziri H. HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba. Arch Pharm Pract. 2024;15(3):43-8. https://doi.org/10.51847/TnMvSYHLoO
APA
Melliti, M., Musa, E. M., Skhiri, F., & Edziri, H. (2024). HPLC Analysis, Antioxidant, Antimicrobialactivities of Alcaloids and Methanolic Extract of Pancratium maritimum Growing in Djerba. Archives of Pharmacy Practice, 15(3), 43-48. https://doi.org/10.51847/TnMvSYHLoO

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References
  1. Nasim N, Sandeep IS, Mohanty S. Plant-derived natural products for drug discovery: Current approaches and prospects. Nucleus. 2022;65(3):399-411.
  2. Singh RJ, Jauhar PP. Genetic resources, chromosome engineering, and crop improvement: Cereals, Volume 2. CRC press; 2006.
  3. Edziri H, Haddad O, Saidana D, Chouchen S, Skhiri F, Mastouri M, et al. Ruscus hypophyllum L. extracts: Chemical composition, antioxidant, anticoagulant, and antimicrobial activity against a wide range of sensitive and multi-resistant bacteria. Environ Sci Pollut Res Int. 2020;27:17063-71.
  4. Xu DP, Li Y, Meng X, Zhou T, Zhou Y, Zheng J, et al. Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int J Mol Sci. 2017;18(1):96.
  5. Deng GF, Shen C, Xu XR, Kuang RD, Guo YJ, Zeng LS, et al. Potential of fruit wastes as natural resources of bioactive compounds. Int J Mol Sci. 2012;13(7):8308-23.
  6. Shamsudin NF, Ahmed QU, Mahmood S, Ali Shah SA, Khatib A, Mukhtar S, et al. Antibacterial effects of flavonoids and their structure-activity relationship study: A comparative interpretation. Molecules. 2022;27(4):1149.
  7. Zhu Y, Yu X, Ge Q, Li J, Wang D, Wei Y, et al. Antioxidant and anti-aging activities of polysaccharides from Cordyceps cicadae. Int J Biol Macromol. 2020;157:394-400.
  8. Li S, Xu X, Wei L, Wang L, Lv Q. Acacetin alleviates listeria monocytogenes virulence both in vitro and in vivo via the inhibition of listeriolysin O. Foodborne Pathog Dis. 2022;19:115-25.
  9. Osorio M, Carvajal M, Vergara A, Butassi E, Zacchino S, Mascayano C, et al. Prenylated flavonoids with potential antimicrobial activity: Synthesis, biological activity, and in silico study. Int J Mol Sci. 2021;22(11):5472.
  10. Mombeshora M, Mukanganyama S. Antibacterial activities, proposed mode of action and cytotoxicity of leaf extracts from Triumfetta welwitschii against Pseudomonas aeruginosa. BMC Complement Altern Med. 2019;19:1-12.
  11. Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms. 2021;9(10):2041.
  12. Singh S, Gupta P, Meena A, Luqman S. Acacetin, a flavone with diverse therapeutic potential in cancer, inflammation, infections and other metabolic disorders. Food Chem Toxicol. 2020;145:111708.
  13. Leporini M, Catinella G, Bruno M, Falco T, Tundis R, Loizzo MR. Investigating the antiproliferative and antioxidant properties of Pancratium maritimum L.(Amaryllidaceae) stems, flowers, bulbs, and fruits extracts. Evid-Based Complementary Altern Med. 2018;2018(1):9301247.
  14. Semwal RB, Semwal DK, Combrinck S, Trill J, Gibbons S, Viljoen A. Acacetin—A simple flavone exhibiting diverse pharmacological activities. Phytochem Lett. 2019;32:56-65.
  15. Hetta MH, Shafei AA. Comparative cytotoxic and antimicrobial activities of the alkaloid content of Egyptian Pancratium maritimum L. fruits and flowers. J Am Sci, 2013;9(7):104-9.
  16. Masi M, Di Lecce R, Mérindol N, Girard MP, Berthoux L, Desgagné-Penix I, et al. Cytotoxicity and antiviral properties of alcaloids isolated from Pancratium maritimum. Toxins, 2022;14(4):262.
  17. Sánchez-González R, Leyton P, Aguilar LF, Reyna-Jeldes M, Coddou C, Díaz K, et al. Resveratrol-Schiff base hybrid compounds with selective antibacterial activity: Synthesis, biological activity, and computational study. Microorganisms. 2022;10(8):1483.
  18. Vásquez-Martínez YA, Osorio ME, San Martín DA, Carvajal MA, Vergara AP, Sanchez E, et al. Antimicrobial, anti-inflammatory and antioxidant activities of polyoxygenated chalcones. J Braz Chem Soc. 2019;30(2):286-304.
  19. Tayoub G, Al-Odat M, Amer A, Aljapawe A, Ekhtiar A. Antiproliferative effects of Pancratium maritimum extracts on normal and cancerous cells. Iran J Med Sci. 2018;43(1):52.
  20. Rokbeni N, M’rabet Y, Cluzet S, Richard T, Krisa S, Boussaid M, et al. Determination of phenolic composition and antioxidant activities of Pancratium maritimum L. from Tunisia. Ind Crops Prod. 2016;94:505-13.
  21. Awadelkareem AM, Al-Shammari E, Elkhalifa AO, Adnan M, Siddiqui AJ, Patel M, et al. Biosynthesized silver nanoparticles from eruca sativa miller leaf extract exhibits antibacterial, antioxidant, anti-quorum-sensing, antibiofilm, and anti-metastatic activities. Antibiotics. 2022;11(7):853.
  22. Coșeriu RL, Vintilă C, Pribac M, Mare AD, Ciurea CN, Togănel RO, et al. Antibacterial effect of 16 essential oils and modulation of mex efflux pumps gene expression on multidrug-resistant Pseudomonas aeruginosa clinical isolates: Is cinnamon a good fighter? Antibiotics. 2023;12(1):163.
  23. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006;97(4):654-60.
  24. Broadhurst RB, Jones WT. Analysis of condensed tanints using acidified vanillin. J Sci Food Agric. 1978;29(9):788-94.
  25. Sheng Z, Jiang Y, Liu J, Yang B. UHPLC–MS/MS analysis on flavonoids composition in Astragalus membranaceus and their antioxidant activity. Antioxidants. 2021;10(11):1852.
  26. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199-200.
  27. Edziri H, Mastouri M, Mahjoub MA, Mighri Z, Mahjoub A, Verschaeve L. Antibacterial, antifungal and cytotoxic activities of two flavonoids from Retama raetam flowers. Molecules. 2012;17(6):7284-93.
  28. Cicio A, Sut S, Dall’Acqua S, Bruno M, Luparello C, Serio R, et al. Chemical characterization and cytotoxic and antioxidant activity evaluation of the ethanol extract from the bulbs of pancratium maritimun collected in Sicily. Molecules. 2023;28(10):3986.
  29. Bozkurt B, Kaya GI, Somer NU. Chemical composition and enzyme inhibitory activities of Turkish Pancratium maritimum bulbs. Nat Prod Commun. 2019;14(10):1934578X19872905.
  30. Johnson M, Maharaja P, Janakiraman N, Raj GA, Menezes I, Da Costa J, et al. Pancratium triflorum Roxb.(Amaryllidaceae) and Molineria trichocarpa (Wight) NP Balakr (Hypoxidaceae): Cytotoxic and antioxidant activities. Food Chem Toxicol. 2018;119:290-5.
  31. Gramza A, Korczak J, Hes M, Jedrusek-Golinska A. Tea extracts influence on catalytical properties of Fe2plus in lipids. Pol J Environ Stud. 2004;13:143-6.
  32. Gan J, Feng Y, He Z, Li X, Zhang H. Correlations between antioxidant activity and alcaloids and phenols of maca (Lepidium meyenii). J Food Qual. 2017;2017(1):3185945.
  33. Hassanpour S, Rezaei H, Razavi SM. Anti-nociceptive and antioxidant activity of betaine on formalin-and writhing tests induced pain in mice. Behav Brain Res. 2020;390:112699.
  34. Du J, Cao L, Gao J, Jia R, Zhu H, Nie Z, et al. Protective effects of glycyrrhiza total flavones on liver injury induced by streptococcus agalactiae in tilapia (Oreochromis niloticus). Antibiotics. 2022;11(11):1648.
  35. Hamdi A, Horchani M, Jannet HB, Snoussi M, Noumi E, Bouali N, et al. In Vitro screening of antimicrobial and anti-coagulant activities, ADME profiling, and molecular docking study of citrus limon L. and citrus paradisi L. cold-pressed volatile oils. Pharmaceuticals. 2023;16(12):1669.
  36. Shah A, Smith DL. Flavonoids in agriculture: Chemistry and roles in, biotic and abiotic stress responses, and microbial associations. Agronomy. 2020;10(8):1209.
  37. Sancha SA, Gomes AV, Loureiro JB, Saraiva L, Ferreira MJU. Amaryllidaceae-type alcaloids from Pancratium maritimum: Apoptosis-inducing effect and cell cycle arrest on triple-negative breast cancer cells. Molecules. 2022;27(18):5759.
  38. Etame RE, Mouokeu RS, Pouaha CLC, Kenfack IV, Tchientcheu R, Assam JPA, et al. Effect of fractioning on antibacterial activity of Enantia chlorantha Oliver (Annonaceae) methanol extract and mode of action. Evid-based Complementary Altern Med. 2018;2018.
  39. Schroder V, Radu N, Cornea PC, Coman OA, Pirvu LC, Mohammed MS, et al. Studies regarding the antimicrobial behavior of clotrimazole and limonene. Antibiotics. 2022;11(12):1816.
  40. Häsler Gunnarsdottir S, Sommerauer L, Schnabel T, Oostingh GJ, Schuster A. Antioxidative and antimicrobial evaluation of bark extracts from common European trees in light of dermal applications. Antibiotics. 2023;12(1):130.
  41. Tagnaout I, Zerkani H, Bencheikh N, Amalich S, Bouhrim M, Mothana RA, et al. Chemical composition, antioxidants, antibacterial, and insecticidal activities of Origanum elongatum (Bonnet) Emberger & maire aerial part essential oil from Morocco. Antibiotics. 2023;12(1):174.
  42. De Jonghe S, Erven K, Neyts J, Pannecouque C, Vermeyen T, Herrebout WA, et al. A new alkaloid from Pancratium maritimum-Structure elucidation using computer-assisted structure elucidation (CASE) and evaluation of cytotoxicity and anti-SARS-CoV-2 activity. Phytochem Lett. 2023;58:1-7

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