Archive \ Volume.15 2024 Issue 1

Impact of Carrageenan-Soy Protein Combination on CXCR-4 Expression, Cell Viability, and Apoptosis in HCT-116 Cells

Sahar El Hadad*✉ , Safiyah Alzahrani , Alawiah Alhebshi , Jehan Alrahimi
  1. Research Center of Genetic Engineering and Bioinformatics, VACSERA, Cairo, Egypt.
  2. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
  3. Department of Basic Medical Sciences, College of Applied Medical Sciences, King Khalid University, Khamis Mishit 61421, Saudi Arabia.
  4. Immunology Unit, King Fahad Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

Abstract

The objective was to explore the therapeutic potential of carrageenan and soy protein in treating colorectal cancer (CRC) by selectively targeting cancer cells and modulating the chemokine receptor CXCR-4. The chemokine receptor CXCR-4 plays a crucial role in colon cancer by promoting tumor cell proliferation, metastasis, and angiogenesis. Therefore, targeting CXCR-4 expression could be a promising approach for colon cancer treatment. We conducted experiments using two groups of HCT-116 cells. The CS-HCT group was treated with a combination of carrageenan and soy protein, while the untreated Group UHCT served as a control. The results demonstrated that the combination treatment with carrageenan and soy protein led to a time-dependent decrease in cell viability compared to the untreated group. The treated group exhibited significantly reduced cell viability, particularly after 48 and 72 hours of treatment. Moreover, the combination treatment induced programmed cell death, as evidenced by increased levels of apoptosis after 48 hours. Interestingly, the expression of CXCR-4 was significantly upregulated in response to the carrageenan/soy protein treatment. However, this increase in CXCR-4 expression was associated with elevated apoptosis levels and reduced cell proliferation. Both apoptosis and cell proliferation were enhanced when CXCR-4 expression levels decreased after 48 and 72 hours. Notably, the highest expression of CXCR-4 was observed at 24 hours of treatment compared to later time points. The results underscore the therapeutic potential of carrageenan and soy protein in colon cancer by targeting CXCR-4 expression, requiring further research for comprehensive understanding.


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Vancouver
El Hadad S, Alzahrani S, Alhebshi A, Alrahimi J. Impact of Carrageenan-Soy Protein Combination on CXCR-4 Expression, Cell Viability, and Apoptosis in HCT-116 Cells. Arch Pharm Pract. 2024;15(1):53-62. https://doi.org/10.51847/CZ6T8kG5lr
APA
El Hadad, S., Alzahrani, S., Alhebshi, A., & Alrahimi, J. (2024). Impact of Carrageenan-Soy Protein Combination on CXCR-4 Expression, Cell Viability, and Apoptosis in HCT-116 Cells. Archives of Pharmacy Practice, 15(1), 53-62. https://doi.org/10.51847/CZ6T8kG5lr

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